MicrofluidicsSP1.0Platform詳細配置如下：▲MicroscopeAxiovert40:Contrastoption:Brightfield,Phase,Varel.Objectives:10xLDA-Plan,20xLDA-Plan,32xCP-Achromat,40xLDA-PlanEyepieces:E-PL10x/20Br.,10x/20Br.foc.Stage:Vena8biochipcompatiblestageholder,Eppendorftubesholder.▲Mirus1.0NanopumpSpecifications:ShearStressRangeof0.05 20dyne/cm2,VolumetricFlowRates100nL 20 L/minute(100 Lsyringe),SampleVolumeIncrementsFreelyadjustableLinearVelocityRangeof10 m/sto10cm/s;FlowDirectionReversible;SampleVolumeAspirationAccuracy 1%;ShearStressAccuracy 0.5%;SampleVolumeAspirationPrecision 1%.Multichannel:16-WaymanifoldallowingindividualaddressingmicrochannelsofVena8biochip.▲MicroscopeCageincubator:Capabilities:Temperaturecontrol 0.10C,CO2andhumiditymodule.Options:Temperaturemonitoringsoftwaremodule,blackpanelsforuseinfluorescentmicroscopy.▲ DeltapixDP200USB2.0cameraSpecifications:1.3MegaPixels(1,280x1,024),ColorCDD,1/2 format,Pixel5.2 mx5.2 m,Resolution:15FPS@1,280x1,024pixels;24FPS@1,204x768pixels;37FPS@800x600pixels;60FPS@640x480pixels.DynamicRange 60dB.▲DellOptiplex745PCcomputerConfiguration:IntelCore2processor;1.86GHz;2GBRAM;ATIRadeonX1300PRO;ObjectiveImagingstagecontrolhardware;19 LCDmonitor.WindowsXPProfessionalSP2;DeltaPixViewerSoftware;FlowAssayandDucoCellsoftwarepreinstalled.▲FlowAssaySoftware.Capabilities:Executionofpresetprotocols.Intuitivestepwiseprotocolinterfacewithstepshierarchyandstatuscontrol.▲DucoCellsoftwareSpecifications:AutomaticCountingandDetection;Morphologicalparametersanalyzedautomatically(Area,diameter,form-factor,ellipticity,eccentricity,orientation,perimeter,asymmetry,cellcentroidcoordinates,ellipticalaxis);Cellsortingandfiltering;Abilitytoanalyzesub-populationsbasedoninclusion/exclusionofmorphologicalparameters.DatamaybeexportedtoExcelspreadsheetincorporatingautomaticgraphgeneration(e.g.%celladhesionvs.shearstress).FileFormatsanalyzed*.jpg,*.jpeg,*.tif,*.tiff,*.bmp,*.gif,*.png.▲Vena8biochipspackof10Specifications:MinimumSampleVolume3 L;MaximumSampleVolume100 L;ShearStressRangeof0.05 20dyne/cm2,stepsof0.05dyne/cm2.Numberofchannelsperbiochipis8;Volumeofeachchannelis0.8 L.Deadvolumeatinputportis0.1 L.Vena8biochipdimensionsare400 m(W)x100 m(D)x20mm(L).